Characterization of DC used for adoptive cellular therapy.

DC transduced with adenoviral vectors expressing soluble IL-4 (DC/sIL-4), membrane-bound IL-4 (DC/mbIL-4), or empty adenoviral vector (DC/ψ5) were compared to non-transduced DC (DC/Non) A.) After harvest on day 8, 1×106 DC were plated per well in OPTI-MEM media a 96-well tissue-culture plate. Following 24 hours of culture, supernatants were analyzed by ELISA for levels of IL-4 secretion. B.) After harvest on day 8, DC were collected and analyzed by flow cytometry. Plots were based on live DC as determined by FSC vs. SSC profiles. Thin lines represent the isotype control stained samples, and bold lines represent staining with anti-B7-1, -B7-2, -PD-L1, or -PD-L2 antibodies, as shown. The percentage of cells in the sample with positive staining is displayed.