Gene expression profile at single cell level of low and high pigmented B16 cells [B16_D7_LP_HP_scRNA]

B16 F10 is a depigmented mouse melanoma cell line which is considered as a homogeneous cell population. These cells when cultured at a very low density of 100 cells/cm2 starts to pigment progressively for a period of 7 days. Phenotypically two broad population of low and high pigmented cells are observed at day 7 in this low density culture. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of B16 cells cultured at low density for 7 days. Overall design: B16 F10 cells were cultured at a very low density of 100 cells/cm2 for 7 days. The low and high pigment cells were sorted using the side scatter intensity of a flow cytometer