Transcription_in_Mecp2_null_and_Cfp1_null_cells
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下载链接:
https://www.ncbi.nlm.nih.gov/sra/ERP001528
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资源简介:
This project aims to assess effects of Cfp1- and Mecp2-deficiency on transcription using RNA-seq of nascent RNA. This involves in vitro run-on transcription with isolated nuclei using BrU as a substrate to permit purification of nascent RNA. This technique shows where active polymerases are loaded on chromatin, unlike conventional RNA-seq, which only detects stable cytoplasmic RNAs.
创建时间:
2021-02-04



