Gene expression and alllele inheritence in xiphophorus spontaneous melanoma in backcross BC1 interspecies animals in Xiphophorus maculatus Skin

RNA-Seq of spontaneous melanoma in Xiphophorus interspecies hybrids, and paired normal skin samples from the same melanoma-bearing animals were performed. Overall design: Gordan-Kosswig cross of X. maculatus-X. hellerii interspecies backcross were made. At age of 9 month, melanoma-bearing animals were identified and dissected. RNA from tumor/skin samples were isolated and sequenced for allelel inheritence analysis and overall gene expression profiling.