Identification of differentially expressed mRNAs in LX-2 cells treated with cholic acid (CA). Identification of differentially expressed mRNAs in LX-2 cells treated with cholic acid (CA)

In this study, high-throughput RNA sequencing technology combined with bioinformatics analysis was used to compare the differentially expressed mRNA of cholic acid-treated LX-2 cells with that of the control group in hopes of gaining a deeper understanding of the biological function and signaling pathway changes in the activation process of LX-2 cells, as well as to identify potential key genes regulating HSC activation. Overall design: LX-2 cells were divided into two groups. The experimental group received 50μM cholic acid stimulation (n=3), while the control group received vehicle treatment (n=3). After 48 hours, total RNA was sequenced by transcriptome to screening for differentially expressed genes (DEGs), and DEGs were bioinformatically analyzed.