Transcriptomic analysis of KDM4B-mediated gene in C4-2B prostate cancer cells

Histone lysine demethylase KDM4B is often overexpressed in prostate cancer and links to poor survival outcome. Recent evidence suggests that KDM4B serves as a potential therapeutic target. The global transcriptomic analysis between control and KDM4B-knockdown C4-2B cells reveals the metabolic genes involved in Warburg effect are downregulated after knocking down the expression of KDM4B, indicating its role in tumor growth. KDM4B control and knockdown cell lines were established in C4-2B cells based on the lentivirus system: LKO (control) and shKDM4B (shRNA-specific RNA) lines. Global expression analysis was then performed (pLKO vs. KDM4B-knockdown). Microarray analysis was conducted by the National Health Research Institutes, using Affymetrix GeneChip Human Gene 2.0 ST Array (Affymetrix, Santa Clara, California, USA).