Bronchoalveolar lavage lung microbiota in children with cystic fibrosis

The goal of this study was to determine the lung microbiota in children with cystic fibrosis, differentiating true lung microbiota from contaminants that could come from the oropharynx, mouth, collection instruments, and/or reagents used to process and sequence samples. Bronchoalveolar lavage samples were collected, as well as throat, mouth, instrument, and reagent controls. DNA was isolated from each sample type and subjected to bacterial 16S rRNA gene sequencing.