Lactate facilitates pancreatic repair following acute pancreatitis by promoting reparative macrophage polarization

Based on the role of lactate in mitigating inflammation during acute pancreatitis (AP) repair, we focused on macrophages, as previous studies have highlighted their essential role in pancreatic tissue repair following AP. To investigate the relationship between lactate and the phenotype and function of macrophages during the recovery phase after AP, we performed high-throughput mRNA sequencing on macrophages isolated from the pancreatic tissues of C57BL/6J wild-type mice, which were administered lactate or PBS following induction of a recovery AP model. Macrophages were isolated from the pancreas of caerulein-treated C57BL/6J wild-type mice on days 0, 1, and 3 following the induction of a recovery AP model, using fluorescence-activated cell sorting. Total RNA was extracted from these macrophages and prepared for RNA sequencing.