Gut microbiota drives vitiligo-related oxidative stress in skin via the metabolite Hippuric Acid

Vitiligo is a common autoimmune skin disorder. We constructed an induced vitiligo mouse model and performed bulk-RNA sequencing on the skin and 16S rRNA sequencing of feces from vitiligo mice and uninduced mice. Next, we performed skin bulk-RNA sequencing after treatment using ABX. Lastly, we subjected gut microbe-related metabolite hippuric acid to control mice and performed bulk-RNA sequencing on the skin to observe oxidative stress-related gene expression changes. Overall design: To mimic the vitiligo skin state, we constructed a vitiligo mouse model and sequenced the tail skin using bulk RNA-seq. To detect the effect of gut microbiota on vitiligo skin, we depleted the gut microbes of vitiligo mice using ABX and tested the skin using bulk RNA-seq. To mimic the effects of elevated concentrations of the gut microbe-derived metabolite hippuric acid on the skin and to rule out other possible factors, we injected hippuric acid intraperitoneally in wild type mice to observe the ROS accumulation phenomenon and sequenced the skin using bulk RNA-seq.