CUT&RUN in SCC-25 Parental and RARG knockout cells maps RAR? binding sites and genome wide changes in H3K4 & H3K27 trimethylation

Vitamin A (retinol) metabolism and signaling through nuclear retinoic acid receptors (RARs a,ß,?) regulate embryonic development, immune functions, and cell differentiation in most cell types. RAR gamma (RAR?) is highly expressed in stratified squamous epithelial cells of the tongue, esophagus, and skin. While data indicate that RAR? agonism is anti-tumorigenic in oral cavity squamous cell carcinoma (OCSCC), the specific, primary gene targets of RAR? remain poorly characterized. Here, we define the transcriptomic consequences of RAR? activity in OCSCC. Using a CRISPR-Cas9-mediated knockout (KO) of RAR? and pharmacological treatments with RAR agonists, we identified RAR?-specific gene targets and pathways, as well as targets that overlap with other RA-responsive nuclear receptors. Overall design: CUT&RUN profiling of SCC-25 Parental (PAR) & RARG knockout (RARGKO) cell lines using antibodies targeting RAR?, H3K4me3, H3K27me3, and IgG. IgG is used as a negative control.