Supplemental Material for Pitchers et al., 2019

Supplemental Table and Figures in PDF format. Supplemental Materials and methods. Supplemental tables in .xlsx format.

File S1. Supplementary Materials and Methods.

File S2. Means and S.D. of PC scores by lab and sex of fly,
eigenvectors of the combined data set, and the estimated among-line genetic
variance-covariance matrix in a 40-dimensional and phenotypic (97-dimensional)
spaces.

File S3. Significant SNPs from
the MANOVA and LASSO analyses.

File S4. Enriched biological
process gene ontology categories. Table
consists of the modified output from the WebGestalt analysis of the 479 genes
implicated by the LASSO-significant SNPs.

File S5. Genes studied in
RNAi knockdown experiments, the quantiles of maximum correlations out of 580
random comparisons, and the angles between effect vectors and the first 5 PCS
of the combined data set.

File S6. Vectors
correlations>0.5 between dictionary knockdown vectors and LASSO SNP vectors.

The File BoythLabs_Wings_28Oct.csv is the Procrustes superimposed data from both labs (22923 wings) from the DGRP used for the primary GWAS described in this study.

The zip file Genotyping-5019.001-01.csv.zip contains all of the genotyping data for the replication analysis for the ME-NC cohort as described in the paper.

slid_lmdata_DGRP+NC&ME.dat contains all of the wings from both the DGRP and the NC & ME replication set jointly superimposed.