Analysis of ddPCR results csv files

Data collection of the detection of intron 3 and intron 12 retaining MAPT species by digital droplet PCR in frontal lateral cortex, hippocampal and cerebellum samples of non-demented individuals and Alzheimer's disease patients. Each retention was detected in a different fluorophore. There are species that retain both introns TIR3+12-MAPT (Fam +, Hex +) which would be translated into DW-Tau (double intron retention), species that retain only intron 3 TIR3-MAPT (Fam +, Hex -) which would be translated into NW-Tau (Nt truncation) or species that retain only intron 12 TIR12-MAPT (Fam -, Hex +) which would be translated into CW-Tau (Ct truncation). Additionally Tubulin-beta and total-MAPT levels were measured by ddPCR to using SYBRGeen for normalyzation purposes.