Transcriptome changes caused by PHF6 knockout in THP1 human AML cell line

CRISPR Cas9 guided knockout (KO) of PHF6 in human THP1 AML cell line. We performed bulk RNA-Seq on knockout (PHF6 KO) and wildtype (CTRL) clones derived from THP1 cells transduced with lentiviral vectors encoding Cas9 protein and either PHF6 gRNAs or non-targeting gRNAs. Our results reveal that PHF6 knockout upregulates self-renewal gene sets and downregulates myeloid differentiation gene sets. RNA-Seq of PHF6 knockout and wildtype THP1 AML cells that were clonally selected and expanded after Cas9-gRNA lentiviral transduction.