Photosensitivity and c-GAS-dependent type I IFN activation in lupus patients with TREX1 deficiency

High throughput screening comparing gene expression in human wildtype and patient (TREX1-mutated) fibroblasts, Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis RNA sequencing of transcripts isolated out of native and 8h post UV-irradiated cell (fibroblast) samples