Nano-ImmunoEra. WP2 T2.1. ECL from Luminol-labelled microbeads on BDD electrode

This folder stores data related to Task 2.1 Carbon-based electrode within Work Package 2 Production Characterization Materials for Improved ECL-based Biosensing, in the NanoimmunoEra Horizon 2020 project. The data includes ECL (Electrochemiluminescence) images and quantitative ECL results about 2.8 micrometer streptavidin-coated magnetic beads functionalized with ammino-derivatized luminol (ABEI) in a 0.1 M sodium carbonate solution. The ABEI is linked to a biotinylated immunoglobulin in a previous functionalization and then attached to the magnetic microbead exploiting the biotin-streptavidin biorecognition. In-situ electrochemical generation of hydrogen peroxide at a boron-doped diamond electrode promoted the chemiluminescence of luminol. The electrochemical production of hydrogen peroxide was achieved in a carbonate solution by an oxidation reaction. At the same time, microbeads labeled with luminol were deposited on the electrode surface. The reported data are useful for a detailed characterization to study the mechanism that brings the luminol emission without its direct oxidation at the electrode surface.