A biphenotypic lymphocyte subset displays both T- and B-cell functionalities

T cell/B cell mixed phenotypic lymphocytes have been observed in different disease contexts, yet their presence and function in physiological conditions remain elusive. Here, we provide evidence for the existence of a lymphocyte subset endogenously expressing both T- and B-cell lineage markers in mice by single cell RNA sequencing. Overall design: Bone marrow, lymph node and peripheral blood cells isolated from C57BL/6J female mice were stained with Live/Dead dye and lymphocytes were isolated using a BDFACSAria™ IIISorter. Cells were manually counted by Trypan blue and AO-PI after each centrifugation and resuspension. Single cells were processed using Chromium Controller (10x Genomics) according to the manufacturer's protocol. By using Chromium Next GEM Single Cell 5' Kit v2 and Chromium Next GEM Chip K Single Cell Kit , we performed single cell TCR/BCR-seq and 5' gene expression profiling.