Single-cell mRNA profiling reveals changes in solute carrier expression and a metabolic switch during zebrafish pronephros development. Single-cell mRNA profiling reveals changes in solute carrier expression and a metabolic switch during zebrafish pronephros development

Developing organisms need to adapt to environmental variations as well as to rapid changes in substrate availability and energy demands imposed by fast-growing tissues and organs. Little is known about the adjustments that kidneys undergo in response to these challenges. We performed single-cell RNA sequencing of zebrafish pronephric duct cells to understand how the developing kidney responds to changes in filtered substrates and intrinsic energy requirements. Overall design: Single cell RNA sequencing was performed on pronephric cells from 1, 2 and 3-day old zebrafish larvae.