Data underlying Chapter 4 of the PhD thesis: An exceptionally high biomass yield on ethanol of the yeast Cyberlindnera jadinii correlates with a mitochondrial localization of key dehydrogenases

Compared to other yeasts, <em>Cyberlindnera jadinii</em> exhibits an exceptionally high biomass yield on ethanol, which is an interesting renewable feedstock for microbial biotechnology. Simulations with a stochiometric metabolic model identified an intramitochondrial rather than cytosolic localization of NAD⁺-dependent alcohol and acetaldehyde dehydrogenases as possible explanation for this high yield. This hypothesis was tested in a comparative study including chemostat cultures in bioreactors, genome and proteome analysis, specific activity of enzymes in subcellular fractions, and mitochondrial staining and imaging using fluorescence.<br>Data used for manuscript in preparation, which is essentially Chapter 4 of the PhD thesis titled Exploring physiological diversity of yeasts for ethanol-fueled industrial biotechnology.

与其他酵母相比，<em>雅津网孢酵母（Cyberlindnera jadinii）</em>在以乙醇为底物时展现出极高的生物量得率；而乙醇本身是微生物生物技术领域极具应用潜力的可再生原料。
借助化学计量代谢模型开展的模拟分析表明，烟酰胺腺嘌呤二核苷酸（NAD+）依赖型醇脱氢酶与乙醛脱氢酶的线粒体内（而非胞质内）定位，可作为该高得率现象的潜在解释。
为验证这一假说，研究团队实施了一项比较性研究，内容涵盖生物反应器中的恒化培养、基因组与蛋白质组分析、亚细胞组分内酶的比活性测定，以及利用荧光技术进行的线粒体染色与成像。
本数据集用于撰写在编学术手稿，该手稿实质上为题为《探索酵母生理多样性以支撑乙醇驱动的工业生物技术》的博士学位论文第4章。