Metabolites confirmed to MSI level 1 in spent media samples that differed by at least 3-fold in intensity between two species.

Key: INF, metabolite absent from spent medium but present in original medium; M, intensity in complete medium; Ldon, intensity in L. donovani spent medium; Lmaj, intensity in L. major spent medium; Lmex, intensity in L. mexicana spent medium; the ratios of the species is based on the ratio for each with the complete medium.*Combined acids co-eluting on ZIC-pHILIC. Separately quantified using C18 AR column.Metabolites confirmed to MSI level 1 in spent media samples that differed by at least 3-fold in intensity between two species.