Gene expression profile of CD4+ CD25- T cells in coculture with regulatory B cells expressing granzyme B (GZMB+Bregs) or total B cells

Regulatory B cells are considered as a very heterogenous population of different origin and functions. Here, we show that Notably, Bregs induced a strong inhibition of T cell genes associated to proliferation, activation, inflammation and apoptosis compared to total B cells. We identified and validated 5 receptor/ligand interactions between Bregs and T cells. Overall design: We used single cell RNA sequiencing (scRNA-seq) to analyze the impact of regulatory B cell expressing GZMB (GZMB+Bregs) or non-Bregs on CD4+CD25- activated T cell transcriptome.