Additional file 1: of Tumor microenvironment modulation enhances immunologic benefit of chemoradiotherapy

Figure S1. The combination of CTX / L-NIL reverses the cold tumor microenvironment. Figure S2. CTX / L-NIL activates the immune microenvironment of CRT treated tumors. Figure S3. CTX/L-NIL improves CRT treatment effects in established HPV-negative tumors. Figure S4. Gating strategy for myeloid sub-types and inflammatory monocyte phenotyping. Figure S5. Systemic myeloid effects induced by CRT+CTX/L-NIL. Figure S6. Gating strategy for lymphocyte sub-types. Figure S7. Systemic lymphoid effects induced by CRT+CTX/L-NIL. Figure S8. CD8+ T cell phenotype in tumors. Table S1. Flow cytometry antibodies used for myeloid subset analysis. Table S2. Flow cytometry antibodies used for lymphocyte and CD8+ T cell subset analysis. Table S3. Immune pathway signature gene list used in gene expression analysis. Table S4. Immune cell type signature gene list used in gene expression analysis [68â 70]. (ZIP 12061 kb)