Functional antibodies induced by the LB-vaccine bind to spirochetes and demonstrate growth inhibiting properties in vitro.

Surface binding and growth inhibition of sera induced with the LB-vaccine (3.0 µg), individual heterodimers (1.0 µg) or the corresponding full-length (FL) OspA protein (1.0 µg) were analyzed by flow cytometry using spirochetes expressing OspA serotypes 1–6 (serotype 1: B. burgdorferi strain ZS7, serotype 2: B. afzelii strain PKo, serotype 3: B. garinii strain Fr, serotype 4: B. bavariensis strain Fin, serotype 5: B. garinii strain PHei and serotype 6: B. garinii strain KL11). The surface binding data are presented as fluorescence titer, which is the reciprocal of the serum dilution which corresponds to a fluorescence intensity of 100. The growth inhibition data are presented as growth inhibition titer, which is the reciprocal of the lowest serum dilution which gives ≥50% reduction in growth as compared to sera from mice immunized with placebo. Not determined (n.d.).Functional antibodies induced by the LB-vaccine bind to spirochetes and demonstrate growth inhibiting properties in vitro.