TIRE-seq: an Integrated Sample Extraction and Transcriptomics Workflow for High Throughput Perturbation Studies

TIRE-seq (Turbocapture Integrated RNA Expression Sequencing) is a novel RNA sequencing method that integrates mRNA purification directly into library preparation, eliminating separate RNA extraction. Compared to traditional protocols like Primeseq, TIRE-seq demonstrates improved sequencing efficiency using crude cell lysates. We validated the method across three biological applications: analyzing transcriptional changes in stimulated human T cells, identifying genes driving murine dendritic cell differentiation, and examining temozolomide's dose-response effects on patient-derived neurospheres. TIRE-seq offers a streamlined, cost-effective approach for gene expression studies, reducing workflow complexity and minimizing sample loss. Overall design: These data contain multiple samples. The biological applications include human T cells stimulated with CD3 and CD28 microbead beads and murine dendritic cells stimulated with Flt3L. Multiple cellular subsets and timepoints are sampled. Samples are bulk RNA-seq but utilise a single-cell style barcoding scheme. See feature_README.txt file at the foot of this record for barcoded sample information.