Poecilia mexicana Transcriptome or Gene expression

We performed an RNA-sequencing study on three different organs collected from fish adapted to hydrogen sulfide (H2S)-rich environments and the absence of light to quantify patterns of gene expression associated with adaptation to extreme environments. We specifically asked: (1) How does gene expression and functional responses change among populations living in different habitats and organs?, (2) Is there evidence of shared expression responses in populations exposed to the same environmental conditions and how do shared responses vary among levels of organization?, and (3) what inferences can analyses of differential expression provide about potential molecular mechanisms underlying adaptation? Overall, we were interested in quantifying transcriptional variation in a unique system that coincides with adaptation to H2S and darkness. Likewise, functional annotations of differentially expressed genes provide a springboard for investigating molecular mechanisms putatively underlying adaptation to extreme environments.