Expression data from mouse perigonadal white adipose tissue - various mutant conditions [exon-level analysis]

Progerin-expressing mice (HGPS-like) demonstrated increased energy metabolism and lipodystrophy. Increased mitochondrial biogenesis was found in adipose tissue from HGPS-like mice, whereas lamin C-only mice had fewer mitochondria. Thus we analyse which molecular pathways mediated the changes in adipose tissue caused by lamin C and progerin expression and the roles of these pathways in energy metabolism and aging. 10 cDNAs samples were analysed. We compared the mean fold change of 2 to 4 biological replicates. We performed an unpaired Student's t-test to compare intensities in the different biological replicates. Genes were considered significantly regulated when the fold change was >= 1.5, and the p-value was<=0.05.表达早老蛋白（progerin）的早衰症样（HGPS-like）小鼠呈现出能量代谢升高与脂肪营养不良表型。该类小鼠的脂肪组织中可见线粒体生物发生水平上调，而仅表达核纤层蛋白C（lamin C）的小鼠，其脂肪组织内线粒体数量更少。据此，本研究拟解析由核纤层蛋白C（lamin C）与早老蛋白（progerin）表达所诱导的脂肪组织改变所介导的分子通路，以及此类通路在能量代谢与衰老进程中的功能作用。本研究共分析10份互补脱氧核糖核酸（cDNA）样本，对2至4份生物学重复样本的平均表达倍数变化值进行比较，并采用非配对Student t检验对不同生物学重复的信号强度进行比较。当基因的表达倍数变化≥1.5且p值≤0.05时，该基因即被认定为存在显著差异表达的基因。