Woodcock, Chris (2010) CIL:714, Necturus maculosus, nucleate erythrocyte. CIL. Dataset

X-ray analysis shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contains more ordered chromatin than most nuclei. Here, chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films and negatively stained with methylamine tungstate. ~30 nm fibers are seen, some exhibiting short regions with apparent structural order.

X射线分析揭示，两栖动物斑马蝾螈（泥狗）的核红细胞中含有比大多数细胞核更为有序的染色质。在此，通过温和的微球菌核酸酶消化，从N. maculosus红细胞核中分离出染色质纤维，并以戊二醛进行短暂固定，随后涂覆于薄碳膜上，并使用甲基胺钨酸盐进行负染色。观察到的纤维直径约为30纳米，其中部分纤维展现出具有明显结构秩序的短区域。