Differentially-expressed genes in blood in response to lipopolysaccharide in three rodent species

Infection tolerance in rodents was examined by injecting single-dose lipopolysaccharide (LPS) to induce inflammation in Peromyscus leucopus (LL stock), the white-footed deermouse also reservoir for Lyme disease and Mus musculus (outbred CD-1 breed), the house mouse, and Rattus norvegicus, the brown rat (Fischer strain). Reaction to LPS was analyzed in the blood of challenged rodents and compared to control animals. As natural reservoirs of zoonoses deermice show significant anti-inflammatory response as described in \"An Infection-Tolerant Mammalian Reservoir for Several Zoonotic Agents Broadly Counters the Inflammatory Effects of Endotoxin\" (https://doi.org/10.1128/mBio.00588-21). The project and the description of the samples are described under the following NCBI BioProjects: PRJNA975149 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA975149) for mouse and deermouse and PRJNA973677 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA973677). This project is a follow-up project focusing on the..., Peromyscus leucopus (10 animals, 5 of each sex) and M. musculus (10 animals, 5 of each sex) were injected into the peritoneum with the solution of purified E. coli lipopolysaccharide (10 microgram/gram of body weight). Same number of animals was injected with saline and used as controls. The rats were studied in a separate experiment with 5 control animals and 6 animals receiving 5 micrograms LPS per gram of body weight and 5 animals receiving 20 micrograms LPS per gram. All animals were euthanized 4 hours post-injection. Blood from each animal was used for extracting RNA and processed further using Novaseq Illumina technology with paired-end chemistry and 150 cycles for mice and deermice and 100 cycles for rats providing ~ 50 million reads per sample. After sequencing reads were trimmed and transcripts annotated using Genomics Workbench v. 23. Differential gene expression (DEG) was assessed using the same suite of software and a modification of EdgeR method. Sequenced data was deposite..., The data is in CSV tabular data format and can be opened as a text file or as a spreadsheet., GENERAL INFORMATION

* Title of Dataset: Differentially-expressed genes in Peromyscus leucopus, Mus musculus, and Rattus norvegicus blood in response to LPS
* Author Information

A. Principal Investigator Contact Information

Name: Alan G. Barbour;
Institution: University of California Irvine;
Address: 843 Health Sciences Court, Irvine, CA 92697;
Email: [e-mail]

B. Associate or Co-investigator Contact Information
Name: Ana Milovic;
Institution: University of California Irvine;
Address: 843 Health Sciences Court, Irvine, CA 92697;
Email: [e-mail]
* Date of data collection (single date, range, approximate date): 2017-2023
* Geographic location of data collection: Irvine, California, USA
* Information about funding sources that supported the collection of the data: National Institute of Allergy and Infectious Diseases grants AI-136523 and AI-157513

SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: None
2. Link...