Total RNA for deep sequencing was isolated by using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol, with some modifications. Each sample added 300 ul TRIzol (I
Elizabethkingia anophelis bacteria encounter fluxes of iron in the midgut of mosquitoes, where they live as symbionts. They also establish bacteremia with severe clinical manifestations in humans, and