YB-1 knock.down - siRNA driven YBX1 knock.down in LNCaP cells in std FBS culture. YB-1 knock.down - siRNA driven YBX1 knock.down in LNCaP cells in std FBS culture

The transcription factor and RNA-interacting Y-box binding protein-1 (YB-1 protein, YBX1 gene) has gained interest as a prognostic biomarker and therapeutic target in various malignancies including prostate cancer. Using a custom prostate-cancer-focussed microarray platform, we have established a transcriptome-wide profile of YB-1 target transcripts in the androgen sensitive prostate cancer cell line LNCaP. Overall design: Gene expression changes in LNCaP prostate cancer cells in standard FBS culture condition after siRNA driven knock down of YBX1 (YBX1-SI) for 48 hrs compared to a non-targeting control siRNA (SC).