Identification of regulatory circRNAs involved in pathogenesis of acute myocardial infarction

The circRNA array was applied to investigate the expression level of circRNAs in the blood samples of coronary arteries of AMI patients and normal persons. Differentially-expressed circRNAs and potential circRNA-miRNA networks were identified in AMI patients. Arraystar human circRNA array was applied to detect and quantify circRNAs in the six samples of three acute myocardial infarction (AMI) patients and three normal controls. Blood samples were collected from the coronary arteries of six participants (three with ST-elevation myocardial infarction (STEMI) and three without AMI) undergoing percutaneous coronary intervention (PCI) due to undiagnosed chest pain. The miRNA expression profiles of AMI patients were identified from a public dataset from Gene Expression Omnibus (GEO) database (GSE31568). The miRNA binding sites on the circRNAs were predicted by miRanda. The miRNA enrichment analysis and annotation tool were used to explore the pathways that the dysregulated circRNAs may participate in.