RNA-seq of mRNAs from spleen and bursa of Fabricius of white leghorn chickens (specific pathogen-free). Gallus gallus

We have used RNA-seq to examine mRNAs from chicken spleen and bursa of Fabricius of three different condition (non-immunized and non-heat-stressed (24 ± 1℃ for 3 h), immunized (Newcastle disease vaccine) and non-heat-stressed, and immunized and heat-stressed (36 ± 1℃ for 3 h)). To clarify how chicken immune systems responded to heat stress with and without immunization. Overall design: To assess mRNA expression during chicken immune systems responded to heat stress with and without immunization, we sequenced and analyzed spleen and bursa of Fabricius at three groups: 1) non-immunized and non-heat-stressed, 2) immunized and non-heat-stressed, and 3) immunized and heat-stressed.