<b>jGCaMP8 transgenic mice</b>

The GENIE Project Team at HHMI Janelia Research Campus have developed and characterized multiple transgenic mice expressing jGCaMP8s and jGCaMP8m and deposited the lines at The Jackson Laboratory (JAX). jGCaMP8s and jGCaMP8m are genetically encoded calcium indicators with fast kinetics (~5 fold shorter rise time) than jGCaMP7f and high sensitivity (2X higher 1AP SNR) than jGCaMP7s (see https://doi.org/10.1038/s41586-023-05828-9 for details). This white paper provides updates on developments in each transgenic mouse line and how to obtain them.The novel mouse lines include:<b>1) Tetracycline‐controlled transactivator protein (tTA)-dependent expression</b>JAX 037717 TetO‐jGCaMP8s<b>2)</b><b> </b><b>Cre recombinase-dependent expression</b>JAX 037718 TIGRE2‐jGCaMP8m‐IRES‐tTA2‐WPREJAX 037719 TIGRE2‐jGCaMP8s‐IRES‐tTA2JAX 037952 TIGRE2-jGCaMP8s-IRES-tTA2-WPREJAX 039267 TIGRE2‐RiboL1-jGCaMP8s‐IRES‐tTA2Browsable 3D histology volumes can be found here.<br>霍华德·休斯医学研究所（HHMI）贾内利亚研究园区的GENIE项目团队开发并鉴定了多种表达jGCaMP8s与jGCaMP8m的转基因小鼠品系，并将这些品系保藏于杰克逊实验室（The Jackson Laboratory, JAX）。jGCaMP8s与jGCaMP8m均为基因编码钙指示剂（genetically encoded calcium indicators），其动力学特性更优异：上升时间较jGCaMP7f缩短约5倍，单动作电位信噪比（1AP SNR）较jGCaMP7s提升2倍，详细信息可参见https://doi.org/10.1038/s41586-023-05828-9。本白皮书针对各转基因小鼠品系的研发进展及获取途径进行了更新说明。新型小鼠品系包括： 1) 四环素调控反式激活蛋白（tetracycline-controlled transactivator protein, tTA）依赖型表达品系：JAX 037717 TetO-jGCaMP8s 2) Cre重组酶（Cre recombinase）依赖型表达品系： JAX 037718 TIGRE2-jGCaMP8m-IRES-tTA2-WPRE JAX 037719 TIGRE2-jGCaMP8s-IRES-tTA2 JAX 037952 TIGRE2-jGCaMP8s-IRES-tTA2-WPRE JAX 039267 TIGRE2-RiboL1-jGCaMP8s-IRES-tTA2 可浏览的三维组织学影像数据集可在此处获取。