Prolonged epigenetic and synaptic plasticity alterations following single exposure to a psychedelic in mice

We examined the effects of the psychedelic drug amphetamine 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) on the epigenomics and transcriptomics in mouse frontal cortex. We conducted ChIP-seq (H3K27ac) and RNA-seq on neurons (NeuN+ fraction) from control mouse brains (mice with only vehicle injection,VEH) and treated mouse brains (24 hours (24h), 48 hours (48h), and 7 days (7d) after amphetamine (2,5-dimethoxy-4-iodoamphetamine [DOI] injection). In these experiments, the frontal lobe of the mouse cortex (anterior to bregma +1 mm) was examined. Each treatment group contained 6 mice. Two replicates were produced on each brain sample for each assay. ChIP-seq data were generated using MOWChIP-seq (Zhu et al., Nature Protocols 14 (2019) 3366-3394.) and RNA-seq data were produced using the combination of SMART-seq2 (Picelli et al., Nature Protocols 9 (2014) 171–181.) and a Nextera XT DNA library preparation kit. The filename contains the information on the experimental group, the number of the mouse within the group, the nubmer of replicate, and the epigenomic/transcriptomic data set (either H3K27ac or mRNA). For example, "VEH1-1-K27ac" refers VEH group mouse 1, replicate 1 ChIP-seq data on H3K27ac.