Gene expression profiles modulated by HES4 transcription factor in established human T-cell leukemias

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive malignancy characterized by an expansion of T-cell progenitors and DNA mutations that lead to an overactive NOTCH1 signaling in over 50% of T-ALL cases. Even though intracellular Notch1(ICN1) can modulate the target gene expression as a monomer, homodimerization of ICN1 is indispensable for the leukemogenesis of mouse T-cells. Hes4 is a direct target of Notch1 dimerization in human T-ALL. To investigate the impact on human T-ALL transcriptome of HES4 expression, we performed a gene expression profiling of CUTLL-1 cell line, transduced with a lentivector encoding HES4, two different clones of shRNA lentiviral constructs against HES4 genes or scramble control.