The effect of different antibody-antigen complexes on sliding velocity (µm s−1) in two different assay solutions.
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Data given as mean ± SEM. Actin-(a-rIgG)-(Rh-rIgG): Actin conjugated with a-rIgG followed by binding of Rh-rIgG. Actin-(a-rIgG)-(BT-rIgG)-SA: complex with BT-rIgG and TRITC-labeled streptavidin (SA). Average velocity based on n experiments (n separate flow cells and experimental occasions). Velocity in each flow cell estimated as in Table 2 No significant difference between any pair of data according to paired t-test (a60; control data excluded) or repeated measures ANOVA (aMC130). Note: study was not designed for high statistical power since small (
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2015-12-02
