Downregulation of ADAM10 induces neuronal differentiation in the ventral spinal cord.

After electroporation at E4, transverse sections at E6 were used for immunostaining. The transfected cells are marked with green fluorescence, and the control morpholinos (Fluo-m) and the untransfected side (left side) serve as controls. The immune reactive cells are stained red. Cell nuclei are labeled by DAPI (blue). (A–X) Immunohistochemistry was performed in adjacent sections with antibodies against Evx1 (A–H), En1 (I–L), MNR2 (M–P), Islet1 (Q–T), and Lim3 (U–X), respectively. Arrows indicate immune reactive cells in the prospective ventricular zone and arrowheads indicate no immune reaction in the transfected region (green). Dotted lines indicate the boundary of the alar plate and the basal plate. (Y–B’) Computer-generated overlay of neurons with different domain markers such as En1 (red) and Evx1 (green) in (Y); En1 (red) and MNR2 (green) in (Z); Lim3 (red) and MNR2 (green) in (A’); and MNR2 (red) and Islet1 (green) in (B’). Arrowheads indicate the expanded cells over the boundary into adjacent domains. Dotted lines indicate the boundary of the different domains. (C’) Quantitative data of the cell number in different domains normalized by the number of the control side, which is set to be 1. All data are presented as mean ± SEM from at least 3 independent experiments (*pp