Genomic binding profiles for ZNF165, ZNF446, and SMAD3 in triple-negative breast cancer

The testis protein ZNF165 is aberrantly expressed in triple-negative breast cancer (TNBC), where it modulates TGFß signaling to promote tumor growth and survival. To investigate whether ZNF165 functions in this capacity via cooperation with SMAD3, a canonical TGFß-induced transcription factor, we performed ChIP-seq for both factors in TNBC cell lines (WHIM12 and SUM159). Additionally, we established genomic binding profiles for ZNF446, a previously uncharacterized transcription factor that we have identified as a member of a ZNF165-SMAD3 complex. Overall design: ChIP-seq for ZNF165, ZNF446, and SMAD3 in WHIM12 and/or SUM159 cells