Results from 3 h and 20 h pairings of experimental males to homozygous wild type 6N females.

1Females were paired with homozygous knockout (KO) or wild type (+, possessing at least one functional allele) males for 3 hours or 20 hours (see text).2Number of matings attempted.3Number (percentage) of pairings for which sperm were observed somewhere in the female reproductive tract.4Number (percentage) of successful crosses that resulted in a copulatory plug.5Number of fertilized and6unfertilized oocytes after 3 hours or 20 hours of pairing. Two pronuclei are only visible after 20 hours of crossing. When analyzed on a per-female basis (see text) Tgm4 knockout males fertilized significantly fewer oocytes than wild type in the 20 hours crosses (t = 3.00, df = 15.14, P = 0.01).