Expression data from cytokine producing human CD4+ T cells

Immune system homeostasis depends on signals that drive effector (like secretion of pro-inflammatory cytokines like IFNg) and regulatory (like secretion of the anti-inflammatory cytokine IL-10) functions. In this study we aimed at understand the signals that drive the switching from IFNg+ to IL-10+ state in CD4+ human T cells Peripheral blood CD4+ T cells were isolated from 9 individuals and stimulated for 36h in the presence of anti-CD3 and anti-CD46 antibodies and IL-10. After incubation, cells were harvested, double IFNg/IL-10 cytokine secretion assay run and IFNg+IL10-; IFNg+IL10+; IFNg-IL10+ and IFNg-IL10- cytokine-producing populations sorted by FACS. Finally, gene expression arrays were performed in the sorted populations