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Gene expression analysis of hematopoietic stem cells based on Syndecan-2 surface expression

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151733
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Analysis of how Syndecan-2 expression identifies hematopoietic stem cells with unique gene expression profiles. We tested the hypothesis that Syndecan-2 expression enriches for hematopoietic stem cell gene expression programs. The results provide important insight into how Syndecan-2 can be used to isolate hematopoietic stem cells with distinct hematopoietic properties. Total RNA was obtained from sorted adult mouse (C57BL/6.J, mixed gender) hematopoietic stem cells isolated using fluorescence activated cell sorting (FACS). The cell populations analyzed are c-Kit+/Sca-1+/Lineage- (KSL) CD34-, KSL CD34- Syndecan-2- (Sdc2m) and KSL CD34- Syndecan-2+ (Sdc2p).
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2022-03-09
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