Epigenomes and Transcriptomes of human monocytes from Hyper IgD Syndrome (HIDS) patients

Innate immune memory, also refered to as trained immunity (TRIM) is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components. In this study we performed epigenomic and transcriptomic analysis of HIDS (Hyper IgD Syndrome) patients at baseline, and after 24 hours culture in media (RPMI only) or media + 10ng/mL LPS. HIDS is associated with the accumulation of mevalonate, a monocyte trainer. HIDS patients were recruited and monocytes were isolated for ChIP-seq and RNA-seq.