Nine protein spots were excised from the 250 mg protein-loaded gel and analyzed by MALDI-TOF MS. A local MASCOT PMF search was performed with its MOWSE-based score (significance threshold score >51, P
In the Chromosome-Centric Human Proteome Project (C-HPP), false-positive identification by peptide spectrum matches (PSMs) after database searches is a major issue for proteogenomic studies using liqu