Analysis of gene expression profiles during tolerance induction in dendritic cells from women with Systemic Lupus Erythematosus

This analysis focuses on identifying differentially expressed genes between samples from patients with lupus (SLE) and healthy controls, using RNA sequencing data. Overview of the Analytical Workflow Quality Control, Preprocessing, and Gene - Performed using the pipeline nf-core/rnaseq. Tools included: FastQC, MultiQC, Trim Galore!, and Salmon. Differential Expression Analysis - Conducted in R using the tximport and DESeq2 packages. Steps included: normalization, model fitting, statistical testing, and correction for multiple comparisons Visualization and Result Export - Generation of publication-ready plots (e.g., volcano plots, heatmaps). Export of results in .csv and graphical formats for downstream interpretation and reporting