The influence of fungal infection on the Drosophila body surface microbiome with different Beauveria bassiana strains

The 2 DPE (days post eclosion) female flies of Drosophila melanogaster W1118 were collected, anesthetized on ice and topically infected with the spore suspensions of the wild type and mutant strains of Beauveria bassiana. The treated insects were kept at a high moisture level for 12h. For each strain treatment,ten flies each were collected again, anesthetized on ice and vortexed with 1 ml PBS buffer for 1 min. The washing buffers were collected and concentrated to 100 microliter by centrifugation. Every four concentrates were pulled together and concentrated to 100 microliter again as one biological repeat. Eight repeats were prepared for each DPE sample. DNA was extracted and amplifed with the 16S V4 primers 515F and 806R. The products were used to generate the amplicon libraries for sequencing with the Illumina Miseq system.