Single-cel RNA-seq of Mouse Olfactory Epithelium for Understanding Age-associated Neuronal Injury Response

This study aims to identify the molecular basis for age-related decline in olfactory function and regeneration. To extend our characterization of olfactory stem cell regeneration during aging, we performed single-cell RNA sequencing on whole olfactory epithelium (OE) of 2-month-old and 18-month-old mice in uninjured state and at 24 hours after injury. Overall design: Whole olfactory epithelium cells are FACS-purified via live cell repoter in young and old adult mice under uninjured (UI) and 24 Hours Post Injury (HPI) condition. A minimum of two biological replicates were collected for each time-point/experimental condition.