Complicated network correlated with Trehalose signaling modulates flowering time of Angelica gigas

Angelica gigas is frequently utilized medicinal plant as a sedative for menstruation. Root part is the major part for pharmaceutical value. To enhance the overall amount of root part of A. gigas, preventing the bolting of plant was key component in A. gigas cultivation. Here, we analyzed bolted samples and juvenile samples from late-bolting clones and early-bolting clones of A. gigas with bulk mRNA sequencing technique. From sequencing data, we could identify four putative mutation to contribute bolting phenotype including homologs for SPA1-RELATED 2, WNK3, Heatshock Protein 17.6, and Histone Acetylase 1 in genic region. Composition of substitution type analysis suggested early-bolting clone was close to be wild-type while late-bolting clones to have altered genotypes. From expression profile, we identified 5,777 genes to be differentially expressed in comparison of bolted sample and juvenile sample from all clones. Functional annotation through gene ontology and GSEA (Gene Set Enrichment Analysis) identified trehalose related signaling to be up-regulated in late-flowering clones of leaf sample in bolted stage. BRG2 and BRG3 (BOI Related Gene2 and 3) were identified in our expression profile to be flowering time correlated regulator gene, which further suggested by co-expression. Our analysis suggests putative genetic background and regulator for flowering time control in A. gigas.