Effects of ambient temperature on DNA accessibility in nuclear chromatin of Drosophila S2R+ cells [ATAC-seq]

Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to ambient temperature change is predicted to involve complex regulation, including the transcriptional level. To study effects of changes in ambient temperature on chromatin accessibility, we performed ATAC-Seq with S2R+ cells, a line derived from embryos of the ectothermic organism Drosophila melanogaster. Aliquots of S2R+ cells were exposed to different temperatures (14, 25 and 29°C) within the readily tolerated range before analysis with ATAC-Seq. Aliquots of S2R+ cells were plated and incubated for 36 hours at 25°C, followed by a shift to either 14, 25 or 29°C. Cells were harvested 24 hours after this temperature shift and processed for tagmentation, which was done at 25°C for all samples. Three replicate experiments were performed.