Single-cell RNA sequencing of human peripheral blood progenitors captures early mast cell differentiation

Activation of mast cells through FcεRI can result in allergic responses. The identity of mast cells progenitors is controversial, in particular whether these cells express FcεRI. Here, we performed single-cell RNA sequencing of human peripheral blood progenitors to study the appearance of FcεRI during hematopoietic progenitor differentiation. We performed single-cell RNA sequencing of FACS isolated human hematopoietic progenitor cells. The first dataset constitutes Lin− c-Kit+ cells sorted from a peripheral blood sample. The second dataset constitutes Lin− CD34+ c-Kit+ cells with spike-in Lin− CD34+ c-Kit+ FcεRI+ cells. Briefly, to generate the latter dataset peripheral blood cells from a donor were divided into two fractions. Cells from one fraction was labeled with phycoerythrin (PE)-hashtag-oligonucleotides. Lin− CD34+ c-Kit+ cells were sorted from the unlabeled fraction and Lin− CD34+ c-Kit+ FcεRI+ cells were sorted from the PE-hashtag-oligonucleotide-labeled fraction. The two sorted cell fractions were pooled and single-cell transcriptomics was performed. **As Swedish legislation and GDPR prevent unrestricted distribution of raw sequencing data, raw sequence data cannot be provided, including through controlled access databases.