Translational efficiency in mock- and heat-treated N.benthamiana leaves

Nicotiana benthamiana is a widely used organism for expression of foreign proteins. To increase protein abundance, overexpression promoters have been developed. However, several proteins cause negative effects on plant growth and development when they are overexpressed. Therefore, overexpression promoters cannot be widely used to generate transgenic plants that produce foreign proteins. In this study, we tried to find stress-inducible promoters which specifically express downstream genes when plants were exposed to stress conditions. By RNA-sequencing analysis of heat- and wound-treated plants, we found that NbHSP20 promoters can be used as a heat-inducible promoters to express foreign proteins in N. benthamiana. The RNA-sequencing data were deposited in GSE210621. In addition to finding mRNA sequencing, we performed polysome RNA sequencing to find genes showing high translational efficiency at high temperature. Three biological replicates of mock- and heat-treated N.benthamiana total RNA and polysome RNA were analyzed