HIV-1 N74D/CA integration targeting into SPADs by vRNP-associated INmNG-CA fusion proteins.

Wild-type (WT) or N74D CA mutant HIV-1 was labeled with Vpr-INmNG or Vpr-INmNG-CA, and the ability of the viral constructs to target SPADs for integration was determined in HEK293T cells at 5 days post infection (MOI 1). As controls, unlabeled virus of respective WT and N74D/CA backbones (noLabel) was used. The total number of unique integration sites, the number of integrations in SPADs, and the % of SPAD targeted integration for each sample is shown. The random integration control (RIC) was calculated in silico. The data shows that vRNP-associated INmNG-CA is unable to stimulate CPSF6-mediated SPAD-proximal integration of N74D virus. (XLSX)